Fig. 5.
Tdh3p regulates intracellular heme availability and Hap1p activity. (A) Fluorimetric determination of EGFP/mKATE2 fluorescence ratios of the indicated yeast strains expressing HS1-M7A. Rmin and Rmax were determined to be 6.1 (from the hem1∆ strain) and 0.2 (from in situ calibration with digitonin), respectively. (B) Hap1p activity in the indicated strains as measured by a transcriptional reporter that used EGFP driven by the CYC1 promoter, a Hap1p target gene. All data represent the mean ± SD of triplicate mid-log-phase yeast extract-peptone-dextrose media supplemented with ergosterol and tween-80 (YPDE) cultures.