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. 2016 Jul 12;6:28117. doi: 10.1038/srep28117

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Competitive index (CI) of fra mutants in vivo. Mixtures (1:1) of wild-type JLD1214 and a mutant (either HMB206 ΔfraB80::kan, HMB184 ΔfraD4::kan, or HMB205 Δfra80::kan) were administered intragastrically (i.g.) to mice that had been treated 24 h earlier with 20 mg of streptomycin. Mice were monitored for survival and four days post-infection ceca were harvested and plated for CFU. Each point is the CI from one mouse with the geometric mean and standard deviation shown. Statistical significance for each group being different than 0 was calculated using a one-sample, two-tailed t-test (****p-value < 0.0001). (B) The raw CFU counts that gave rise to the CI were log transformed and plotted (limit of detection of 300 CFU). Error bars show the mean and standard deviation (****P < 0.0001).