Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 11;6:29425. doi: 10.1038/srep29425

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) General workflow of generation of MLV Gag based VLPs. (B) Schematic representation of the purification protocol. (C) Western blot analysis of VLPs. Particles obtained with ultracentrifugation through 20% sucrose cushion were further centrifuged through stepwise sucrose density gradient (20%, 35%, 45%, 60%) at 120 000 g and 4 °C for 1.5 h in a Beckman SW55 rotor and divided into 10 fractions. The presence of VLPs in each fraction was analyzed by specific antibodies against melanoma antigens and the MLV Gag protein. (D) Physical characterization of VLPs ultracentrifuged through 20% sucrose cushion as assessed by DLS. The mean value of 4 × 10 measurements performed at 22 °C is shown. (E) Transmission electron micrograph of negatively stained MAGEA4 VLPs purified through density gradient centrifugation. Enlarged image of particle is shown on the upper-right corner of the picture.