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. 2016 Jul 11;6:29425. doi: 10.1038/srep29425

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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FACS analysis was performed with VLPs bound to aldehyde/sulfate latex beads as described in Materials and Methods section. The red line corresponds to VLPs carrying recombinant melanoma antigens, the blue line to VLPs without cancer antigen, and grey area shows the signal obtained with secondary Alexa488-labelled antibody. One representative experiment out of the three performed is shown. MFI = Mean fluorescence intensity.