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. 2016 Jul 11;6:29425. doi: 10.1038/srep29425

Figure 3. Subcellular localization of melanoma antigens used in this study.

Figure 3

The COP5 cells transfected with plasmids encoding for the MLV Gag protein and respective cancer antigen were grown on cover slips, fixed and incubated with specific antibodies and Alexa568-conjugated secondary antibody as described in Methods section. Untransfected cells (mock control) incubated with specific antibodies are shown. DAPI was used to stain nuclei of the cells.