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. 2016 Jul 11;6:29370. doi: 10.1038/srep29370

Figure 3. Effect of LeftyA on Rac1 transcript levels and activity in Ishikawa cells.

Figure 3

(a) Arithmetic means ± SEM (n = 4) of Rac1 normalized to L19 transcript levels in human endometrial cancer Ishikawa cells following treatment with LeftyA (25 ng/ml) for 2 hours. Data are depicted as fold induction relative to transcript levels of untreated samples. (b) Representative original Western blots showing activated Rac1 and total Rac1 protein abundance in human endometrial cancer Ishikawa cells after 2 hours culture in the absence or presence of LeftyA (25 ng/ml). (c) Arithmetic means ± SEM (n = 4, arbitrary units) of phospho-Rac1 protein ratio normalized to GAPDH in Ishikawa cells after 2 hours culture in the absence or presence of LeftyA (25 ng/ml). *P < 0.05; **P < 0.01; ***P < 0.001 using Student’s t-test.