Table 3. Primers and RNAs used in this study.
| Category | Primer and RNA name | Sequence (5′-3′) |
|---|---|---|
| RNAs | cgi-miR-2d mimics | UAUCACAGCCUGCUUGGAUCAGU UGAUCCAAGCAGGCUGUGAUAUU |
| Positive control | GUGUAUCACAGAAAAUCCCU GGAUUUUCUGUGAUACACUU | |
| miRNA control | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT | |
| cgi-miR-2d inhibitor | ACUGAUCCAAGCAGGCUGUGAUA | |
| UTR clone primers | CgIκB2_UTRclone_F | TATTACAATCTGGTGCCGATGTGA |
| AP-dT | GGCCACGCGTCGACTAGTACT17 | |
| CgIκB2_mutate_F | AGCTGACGACGACACTATGGCTGATTG | |
| CgIκB2_mutate_R | ACAATCAGCCATAGTGTCGTCGTCAGC | |
| Recombination primers | CgIκB2 _ XhoI | CTCGAGAGAAAGGACTGCCGAA |
| CgIκB2 _ NotI | GCGGCCGCTTTAGACTTGTC | |
| RNAi clone primers | siCgIκB2 _basic_F | AGACCCATGCAAAATCTGGAC |
| siCgIκB2 _basic_R | CTTCTTCCTCCTCCGATTCATC | |
| siCgIκB2_T7_F | TAATACGACTCACTATAGGGATC AGACCCATGCAAAATCTGGAC | |
| siCgIκB2_T7_R | TAATACGACTCACTATAGGGATC CTTCTTCCTCCTCCGATTCATC | |
| siEGFP_basic_F | CGACGTAAACGGCCACAAGT | |
| siEGFP_basic_R | CTTGTACAGCTCGTCCATGC | |
| siEGFP_T7_F | TAATACGACTCACTATAGGGA TCCGACGTAAACGGCCACAAGT | |
| siEGFP_T7_R | TAATACGACTCACTATAGGGATC CTTGTACAGCTCGTCCATGC | |
| Real-Time primers | GAPDH_F | AGGTCGGTGTGAACGGATTTG |
| GAPDH_R | TGTAGACCATGTAGTTGAGGTCA | |
| EF-F | AGTCACCAAGGCTGCACAGAAAG | |
| EF_R | TCCGACGTATTTCTTTGCGATGT | |
| CgIκB1 _RT_F | CCCTTCACATTGCCAGTAG | |
| CgIκB1 _RT_R | ATTGGGAGATGGGTGTTCT | |
| CgIκB2 _RT_F | CGAGTGATGAATCGGAGGAGG | |
| CgIκB2 _RT_R | CACACAATCAGCCAATCACAGC | |
| CgIκB3 _RT_F | ACCTCCCCTCCCTACAACCTCAGACT | |
| CgIκB3 _RT_R | CCTGGTGACATGGAATGGGCAACT | |
| cgi-miR-2d | TATCACAGCCTGCTTGGATCAGT | |
| rRNA_5s | CAAGGATGACACGCAAAT |