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. 2016 Jul 12;6:29588. doi: 10.1038/srep29588

Figure 2. Effect of ONA on tumour cell proliferation in co-culture of EOC cells with HMDMs.

Figure 2

Compared with EOC mono-culture (A-i), non-treated (A-ii) and onionin A (ONA) (10 μM)-treated HMDMs (A-iii) were incubated with epithelial ovarian cancer (EOC) cells (SKOV3, ES2, and RMG1) for 24 hours, followed by the determination of BrdU-positive cells by ELISA (B). Compared with EOC mono-culture (C-i) and co-culture without ONA treatment (C-ii), co-cultured cells (HMDMs and EOC cells) were treated with the indicated concentrations of ONA (3–10 μM) for 24 hours (C-iii), followed by the determination of BrdU-positive cells by ELISA (D). SKOV3 cells were labelled with CytoTell Red fluorescence and co-cultured with HMDMs, and then cell proliferation was assessed by flow cytometry (E). IL-10 production was evaluated by ELISA (F). HMDM-derived soluble factors were evaluated by a cytokine array kit, and the relative spot densities of cystatin C, macrophage migration inhibitory factor (MIF), growth differentiation factor (GDF)-15, urokinase plasminogen activator receptor (uPAR), matrix metalloproteinase 9 (MMP9), epidermal growth factor (EGF), and IL-1 receptor antagonist (IL-1Ra) were evaluated using the ImageJ software programme (G). The data are presented as the mean ± SD. *p-value < 0.05, **p-value < 0.01 vs. control.