Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 11;6:29514. doi: 10.1038/srep29514

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Western blotting analysis of EURL protein levels (represented as an immunoreactive band of approximately 34kDa) in brain lysates over the course of mouse brain development, from embryonic (E14.5, E17.5) to postnatal (P0, P2, P5, P17, P30). Signals for β-tubulin (approximately 50kDa in size) serve as loading control. (B) Immunostaining reveals prominent EURL reactivity (green signal) within the dorsal telencephalon (region magnified in “*”) as well as the developing retina and lens (region magnified in “**”). Double immunostaining with the early neuronal marker TUJ1 (red) reveals EURL immunoreactivity in neural and non-neural cells. (C) Within the E14.5 dorsal telencephalon, EURL immunoreactivity is strong in posmitotic cells of the cortical plate (CP) and marginal zone (MZ), with a weaker signal detected in cells of the intermediate zone (IZ) and germinal subventricular/ventricular zone (SVZ/VZ). A CP neuron is represented with an arrow in the boxed insert. (D) At late embryonic (E17.5) stages, EURL immunoreactivity persists in CP neurons, as well as in postnatal (P0, P5) brains. The immunostaining signals detected with the EURL antibody are not evident when a parallel experiment is conducted with preimmune serum. Scale bar, 200 μm.