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. 2016 Jul 11;6:29514. doi: 10.1038/srep29514

Figure 5. Knockdown as well as over-expression of Eurl alters the long-term positioning of cortical projection neurons within the postnatal (P17) cerebral cortex.

Figure 5

(A) Treatment with Eurl shRNAs leads to suppression of FLAG-EURL levels in transiently transfected P19 cells, visualised by Western blotting. (B) Treatment with Eurl shRNAs impaired the positioning of E14.5-born, GFP-labelled cortical projection neurons, as determined by the proportion of cells within the cortex which is divided into 8 arbitrary bins (F7,32 = 5.5, P = 0.0003). (C) Forced expression of Eurl also disrupted the long-term positioning of E14.5-born cortical projection neurons (F7,32 = 5.5, P < 0.0001). (D) The migration profile of cells treated with control shRNA (scr) vector, or GFP-only control vector was not significantly different (F7,32 = 2.0, P = 0.0838). At least 700 cells were counted from 3 brains per condition. Two-way ANOVA analysis was performed in each case, followed by a Bonferroni posthoc test with multiple correction testing, *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 200 μm.