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. 2016 Jul 11;6:29514. doi: 10.1038/srep29514

Figure 7. EURL interacts with CCDC86B in vitro and influences β-catenin levels in HEKT293T cells.

Figure 7

(A) Co-immunoprecipitation experiments with a FLAG-tagged EURL polypeptide confirm an interaction with GFP-CCDC85B fusion protein, and not GFP alone. Input lanes confirm all proteins are expressed in each condition tested. (B) In HEK293T cells, forced expression of CCDC85B leads to a reduction in myc β-catenin levels in transient transfection assays. (C) Forced expression of Eurl leads to an increase in myc β-catenin levels. (D) Forced expression of Ccdc85b suppresses myc β-catenin, but co-delivery of Eurl abrogates this effect. Quantification of immunoblotting signals was performed on three independent experiments.