Figure 3. Calpain-9, but not calpain-8, attenuates subcutaneous tumor growth in nude mice.

Subcutaneous tumor models were established described in the “Materials and Methods” section. After sacrifice of the mice, tumor xenografts were collected. (a) Representative images of tumors in three groups. (b) Tumor weight in each group was evaluated. (c) The cleavage of caspase-12 in tumor tissues was determined by western blot. (d,e) Immunohistochemistry staining was performed to detect Ki-67 expression (d) and TUNEL assay was performed to detect cellular apoptosis (e). The gels were run under the same experimental conditions. Full-length blots are presented in the Supplement. Scale bar, 50 μm. In (b,d,e) the statistics were made by comparing with vector group, respectively. **P < 0.01; ***P < 0.001; n.s., no significance.