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. 2012 Jun 21;7(3):340–348. doi: 10.1111/j.1750-2659.2012.00396.x

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© 2012 Blackwell Publishing Ltd

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Mean serum hemagglutination inhibition (HI) titers (log2) induced in specific pathogen‐free (SPF) chickens after a single dose of virus‐like particle (VLP) vaccine with ISA70 adjuvant. A total of 32 five‐week‐old SPF chickens (8 per group) were intramuscularly immunized with HPAI H5N1 VLP vaccines. HI titers against the homologous antigen (A) A/chicken/Korea/ES/2003 (clade 2·5) and heterologous antigen (B) A/Indonesia/5/2005 (clade 2·1) and (C) A/Vietnam/1194/2004 (clade 1) were determined 3 weeks after vaccination. Mock – emulsified solution of conditioned SF900III medium with ISA70. *P < 0·05 and ***P < 0·001 by anova with Tukey–Kramer post‐test compared with other groups.