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(A) Lane 1: His6‐NB1; Lane 2: co‐purification of untagged GFP with His6‐NB1 from E. coli cell lysate; Lane 3: co‐purification of untagged GFP with His6‐pcNB1; Lane 4: His6‐GFP; Lane 5: His6‐pcNB1. (B) Tube 1: His6‐NB1; Tube 2: His6‐pcNB1; Tubes 3–4: His6‐NB1 and co‐eluted GFP; Tubes 5–6: His6‐pcNB1 and co‐eluted GFP; Tube 7: His6‐GFP; Tube 8: untagged GFP. For all figures, experiments were run in triplicate and representative data are shown.
