Figure 4.

Renal DPPIV activity and expression in sham and HF rats treated with the DPPIV inhibitor vildagliptin or with the vehicle. (A) Renal DPPIV activity was measured in sham and HF rats treated with vehicle (HF) or vildagliptin (HF + IDPPIV) by colorimetry. (B) Renal cortical DPPIV abundance was evaluated by immunoblotting. Equal amounts of protein (10 μg for DPPIV and 5 μg for actin) were subjected to SDS-PAGE, transferred to a PVDF membrane and incubated with the following primary antibodies: anti-DPPIV (1:1000) and anti-actin (1:50,000). Membranes were stained with Ponceau S prior to antibody incubation, and the Actin was used as an internal control. Values are means ± SEM. ^##p < 0.01 vs. HF.