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. Author manuscript; available in PMC: 2016 Jul 12.
Published in final edited form as: Methods Enzymol. 2016 Feb 16;573:387–401. doi: 10.1016/bs.mie.2016.01.019

Fig. 3.

Fig. 3

The effect of NaCl on binding affinity. The DNA-binding affinity of WT1 + KTS isoform is uniformly low in 300 mM NaCl (A) but increases markedly in 200 mM NaCl (B). (C) The DNA-binding affinity (KD) of human PRDM9 allele-A under NaCl concentrations from 260 to 310 mM with 10–20 mM increments. (D) The linear correlation of double-logarithmic plot of KD values and NaCl concentrations. Panel (B): Adopted from Hashimoto, H., Olanrewaju, Y. O., Zheng, Y., Wilson, G. G., Zhang, X., & Cheng, X. (2014). Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence. Genes & Development, 28, 2304–2313.