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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: ACS Nano. 2016 May 16;10(6):6008–6019. doi: 10.1021/acsnano.6b01560

Figure 5. Assessment of the pro-fibrogenic effects of the electronic sorted SWNCTs in mice.

Figure 5

Figure 5

Figure 5

Anesthetized C57BL/6 mice were exposed to SWNCTs, delivered by one-time oropharyngeal aspiration of a 2.0 mg/kg bolus dose. Animals were euthanized after 21 d, and BALF was collected to determine (A) TGF-β1 and (B) PDGF-AA levels. (C) Assessment of total collagen content by a Sircol kit (Biocolor Ltd., Carrickfergus, UK). *p < 0.05 compared to control. (D) Visualization of collagen deposition in the lung, using Masson’s trichrome staining. Collagen deposition is shown as blue staining under 100 × magnification. Animals exposed to Quartz (QTZ) served as positive control.