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HepG2 cells were cultured overnight in medium containing 1 mmol/l glucose and 50 μg/ml SN50, an NF-κB inhibitor, followed by treatment with 30 mmol/l glucose for 6 h. Glucose-mediated increase in pri-miR-425 levels is abrogated in the presence of SN50. pri-miR-425 levels are relative to those in untreated cells (Control). Data are expressed as mean ± SEM (n = 6).
Abbreviations as in Figure 5.
