1920 x 1920 image size

16-bit

Pivot scan on

Dual sided illumination with online fusion

10X/0.2 illumination objective

20X/1.0 W Plan-Apochromat detection objective

Track 1: Excitation 488 nm typically 2% of 100 mW laser, 550 nm SP emission filter

Track 2: Excitation 561 nm typically 3% of 75 mW laser, 585 nm LP emission filter

Exposure time up to 100 msec

Z stack thickness 50-100 μm

1-1.5 μm z step size in the continuous z drive mode

Incubation at 28.5 °C