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. 2016 Apr 11;(110):53249. doi: 10.3791/53249
Linearity LOQ
a Slopea Offseta µM
without matrix 0.99999 0.0223 -0.0019 1
1:10 diluted matrix  0.99999 0.0221 -0.0011 1
Standard Meanb Precisionb Accuracyb
µM µM CV% Bias (%error)
without matrix 50 49.96 3.05 -0.09
1 1.04 2.95 3.86
1:10 diluted matrix  50 49.98 0.44 -0.04
1 1.00 4.58 0.33
a Mean of three measurements, calibration with six concentrations of pyruvate from 1 to 50 µM.
b (n = 3) 
LOQ: limit of quantification, CV: coefficient of variation.