Linearity | LOQ | |||
r²a | Slopea | Offseta | µM | |
without matrix | 0.99999 | 0.0223 | -0.0019 | 1 |
1:10 diluted matrix | 0.99999 | 0.0221 | -0.0011 | 1 |
Standard | Meanb | Precisionb | Accuracyb | |
µM | µM | CV% | Bias (%error) | |
without matrix | 50 | 49.96 | 3.05 | -0.09 |
1 | 1.04 | 2.95 | 3.86 | |
1:10 diluted matrix | 50 | 49.98 | 0.44 | -0.04 |
1 | 1.00 | 4.58 | 0.33 | |
a Mean of three measurements, calibration with six concentrations of pyruvate from 1 to 50 µM. | ||||
b (n = 3) | ||||
LOQ: limit of quantification, CV: coefficient of variation. |