Spin No.	Speed (rpm)	Valve No.	Time (sec)	Operation
1	3,600		60	blood separation
2	2,400	1	3	open valve to transfer the plasma into the chamber containing 8 µl of detecting antibodies conjugated with HRP
3	15 Hz, 15°		5	mix plasma and detecting antibodies
4	2,400	2	3	open valve to transfer the mixture into binding reaction chamber
5	60 Hz, 2°		1,200	mix capture antibodies on TiO2 NF mat and plasma-detecting antibodies mixture
6	2,400	3	10	open valve to remove the mixture
7	2,400	4	4	open valve to transfer washing buffer
8	30 Hz, 30°		120	mix washing buffer and wash TiO2 NF mat
9	2,400		4	transfer washing buffer
10	30 Hz, 30°		120	mix washing buffer and wash TiO2 NF mat
11	2,400		4	transfer washing buffer
12	30 Hz, 30°		120	mix washing buffer and wash TiO2 NF mat
13	2,400		4	transfer washing buffer
14	30 Hz, 30°		120	mix washing buffer and wash TiO2 NF mat
15	2,400		20	remove the remaining washing buffer
16		5		close valve
17	2,400	6	10	open valve and transfer the chemiluminescent substrate
18	30 Hz, 2°		60	mix substrate and the immunoreagents on TiO2 NF mat
19	2,400	7	10	open valve and transfer the reacted substrate to the detection chamber
Total time	~ 30 min