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. 2016 Apr 20;(110):52589. doi: 10.3791/52589
Antibody Staining Concentration Time Temperature
DCs Mix
CD11c PE-Cy7 2 µg/ml 30 min 4 °C
MHC II PE " " "
CD45 PE-Cy5.5 " " "
CD207 APC " " "
Macrophages Mix
F4/80 APC-Cy7 2 µg/ml 30 min 4 °C
MHC II PE " " "
CD45 PE-Cy5.5 " " "
CD64 PE-Cy7 " " "
T and B cells Mix
CD8a PE 2 µg/ml 30 min 4 °C
CD4 APC-Cy7 " " "
CD19 APC " " "
CD45 PE-Cy5.5 " " "