Antibody | Staining | Concentration | Time | Temperature |
DCs Mix | ||||
CD11c | PE-Cy7 | 2 µg/ml | 30 min | 4 °C |
MHC II | PE | " | " | " |
CD45 | PE-Cy5.5 | " | " | " |
CD207 | APC | " | " | " |
Macrophages Mix | ||||
F4/80 | APC-Cy7 | 2 µg/ml | 30 min | 4 °C |
MHC II | PE | " | " | " |
CD45 | PE-Cy5.5 | " | " | " |
CD64 | PE-Cy7 | " | " | " |
T and B cells Mix | ||||
CD8a | PE | 2 µg/ml | 30 min | 4 °C |
CD4 | APC-Cy7 | " | " | " |
CD19 | APC | " | " | " |
CD45 | PE-Cy5.5 | " | " | " |