Synthetic Defined (SD) Minimal Yeast Medium	2% dextrose, 0.67% yeast nitrogen base without amino acids, 0.002% adenine, 0.004% uracil, 0.002% arginine, 0.001% histidine, 0.006% isoleucine, 0.006% leucine, 0.004% lysine, 0.001% methionine, 0.006% phenylalanine, 0.005% threonine, 0.004% tryptophan. For solid (plate) medium, include 2% agar.	1. Selective medium is prepared by omitting appropriate amino acid(s) or nitrogenous bases.
2. For convenience, these ingredients may be maintained as concentrated stock solutions as follows. Amino acids may be maintained as 100x stock solution containing all desired amino acids. Yeast nitrogen base may be maintained in a 20x stock solution (13.4%). Dextrose may be maintained in a 40% stock solution. Adenine and uracil may be maintained as 1% stock solutions in 0.1 M NaOH.
3. Sterilize medium by autoclaving.
Yeast Extract-Peptone Dextrose (YPD) Rich Yeast Medium	1% yeast extract, 2% peptone, 2% dextrose (Optional: 0.002% adenine). For solid (plate) medium, include 2% agar.	1. To prevent the slow growth and red coloration characteristic of particular laboratory yeast strains in the absence (or low abundance) of adenine, adenine may be added to YPD growth medium.
2. For convenience, some ingredients may be maintained as concentrated stock solutions as follows. Dextrose may be maintained in a 40% stock solution. Adenine may be maintained as a 1% stock solution in 0.1 M NaOH.
3. Sterilize medium by autoclaving.
20x Stop Mix	200 mM sodium azide, 5 mg/ml bovine serum albumin	Sodium azide is toxic via oral ingestion or dermal contact. Follow manufacturer recommendations when preparing, storing, and handling sodium azide. In the event of accidental exposure, consult material safety data sheet provided by manufacturer.
20 mg/ml Cycloheximide		1.  Prepare in ethanol. Store at -20 °C.
2. Cycloheximide is a dermal irritant and is toxic via oral ingestion. Follow manufacturer recommendations when preparing, storing, and handling cycloheximide. In the event of accidental exposure, consult material safety data sheet provided by manufacturer.
0.2 M Sodium Hydroxide		Prepare in water. Sodium hydroxide reacts with glass. Therefore, for long-term storage, 0.2 M sodium hydroxide should be maintained in plastic containers.
Laemmli Sample Buffer	2% SDS, 10% glycerol, 5% β-mercaptoethanol, 60 mM Tris HCl pH 6.8, 0.008% bromophenol blue	1. Laemmli Sample Buffer is often prepared by diluting a more concentrated (e.g., 5x) stock.
2. The dye bromophenol blue may be added to desired intensity. A "pinch" (very small amount tapped from the edge of a spatula) is typically sufficient.
Laemmli Running Buffer (5x)	125 mM Tris, 960 mM glycine, 0.5% SDS	To prepare 1 L of 1x Laemmli Running Buffer, dilute 1:5 in dH2O.
Tris Acetate-SDS Transfer Buffer (5x)	125 mM Tris acetate (pH 8.8), 960 mM glycine, 0.05% SDS	To prepare 20 L of 1x Tris Acetate-SDS Transfer Buffer, combine 4 L of 5x stock, 4 L of methanol, and 12 L of dH2O.
10x Tris-Buffered Saline (TBS)	500 mM Tris, 1.5 M NaCl; pH adjusted to 7.5	To prepare 1 L of 1x TBS, dilute 1:10 in dH2O. 1x TBS may be supplemented with the detergent Tween-20 and powdered skim milk, as appropriate.