Table 10.

Sample conversion of total [³H]-DTG counts into nanomolar concentrations in assay

Targeted [3H]-DTG concentration (nM)	Measured total radioactivity (DPM)	Measured total [3H]-DTG (mmol)	Measured [3H]-DTG concentration in assay (nM)
300	2337238	2.63E-08	310
100	693785.5	7.81E-09	92
30	204121.8	2.30E-09	27
10	67841.83	7.64E-10	9
3	20148.28	2.27E-10	2.7
1	6984	7.86E-11	0.8
0.3	2223	2.50E-11	0.25

The first column displays targeted concentrations of [³H]-DTG calculated from the stock vial (40 Ci/mmol or ~30 μM). Second column contains radioactivity counts in DPM of the targeted concentration of [³H]-DTG. Third column shows radioactivity values transformed from DPM into millimoles of radioligand (Note: the conversion factor used was 2.22 × 10¹² DPM per Ci). The fourth column shows the actual concentrations of [³H]-DTG as calculated from the DPM values obtained.