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. Author manuscript; available in PMC: 2016 Dec 8.
Published in final edited form as: Curr Protoc Pharmacol. 2015 Dec 8;71:1.34.1–1.3421. doi: 10.1002/0471141755.ph0134s71
Total binding (μL) Non-specific (μL) Inhibitor (μL)
GPL (0.4 mg/ml) 80 80 80
100 nM [3H]-(+)-pentazocine 10 10 10
100 μM haloperidol - 10 -
10X Inhibitor (concentrations vary, see Table 6) - - 10
Assay buffer 10 - -
Total 100 100 100