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. Author manuscript; available in PMC: 2016 Dec 8.
Published in final edited form as: Curr Protoc Pharmacol. 2015 Dec 8;71:1.34.1–1.3421. doi: 10.1002/0471141755.ph0134s71
Total binding (μL) Non-specific (μL) Inhibitor (μL)
RL membranes (0.4 mg/ml) 70 70 70
0.30 μM or 300 nM [3H]-DTG 10 10 10
100 μM haloperidol - 10 -
1 μM (+)-pentazocine 10 10 10
10X Inhibitor (concentrations vary) - - 10
Assay buffer 10 - -
Total 100 100 100