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. Author manuscript; available in PMC: 2016 Dec 8.
Published in final edited form as: Curr Protoc Pharmacol. 2015 Dec 8;71:1.34.1–1.3421. doi: 10.1002/0471141755.ph0134s71

Table 4.

Sample saturation of [3H]-(+)-pentazocine binding data

Bound [3H]-(+)-pentazocine (pmol/mg)
Measured [3H]-(+)-PTZ concentration (nM) Total Nonspecific Specific
270.0 7.56 2.43 5.13
90.0 7.43 2.39 5.04
27.1 5.15 0.82 4.33
8.8 3.23 0.28 2.95
2.8 1.88 0.11 1.77
0.9 0.77 0.09 0.68
0.3 0.52 0.04 0.48

Total binding is defined as [3H]-(+)-pentazocine bound while nonspecific binding is the counts obtained for samples that contain 10 μM haloperidol. Specific binding is defined here as total – nonspecific.