Table 7.

Sample transformation of competition binding against [³H]-(+)-PTZ

Final unlabeled inhibitor concentration (M)	Specific [3H]-(+)-PTZ Bound (DPM)	% Specific Binding
1.00E-05	851	5.25
3.00E-06	950	6.06
1.00E-06	1250	8.51
3.00E-07	3521	27.10
1.00E-07	5420	42.64
3.00E-08	8210	65.47
1.00E-08	10886	87.37
3.00E-09	11253	90.37
1.00E-09	12011	96.57
3.00E-10	12296	98.91

The first column displays the concentrations of unlabeled inhibitor as determined by serial dilution (Table 6). The second column shows mean of quadruplicate counts (in DPM) measured. Values in the third column are derived using Equation 3 where the nonspecific binding value was 209 DPM and the total binding was 12220 DPM.