| Component | 1× volume (μl) | Final concentration (mM) |
|---|---|---|
| 1.5 M sucrose | 2,500 | 250 |
| 1 M KCl | 2,250 | 150 |
| 1 M MgCl2 | 450 | 30 |
| 1 M Tris buffer, pH 8.0 | 900 | 60 |
| Nuclease-free water | 8,900 | — |
| Total volume | 15,000 | — |
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