| Component | 1× volume(μl) | Final concentration |
|---|---|---|
| 10% (vol/vol) Triton X-100 | 20 | 0.2% (vol/vol) |
| RNase inhibitor 40 U μl−1 | 50 | 2 U μl−1 |
| ERCC spike-in mix 1, 1:2,000 | 1 | 1:2 × 106 |
| Nuclease-free water | 929 | — |
| Total volume | 1,000 |
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