Table I.

Summary of Validation Parameters for Analysis of 25-C-NBOMe by HPTLC

Parameter	Results
Rf	0.37
Linearity and range	19.72–118.28 µg per band; y = 16.76X − 16.23, R2: 0.99772, SD: 3.64
LOD and LOQ	7.1 µg per band, and 21.63 µg per band
Repeatability (50.2 µg)	CV% = 5,459
Intermediate precision	CV% = 6,071
Amount of 25-C-NBOMe (µg/mL)	Recovery (%)
18,825	95,635 ± 0.389
31,375	97,098 ± 1,166
43,925	102,213 ± 2,507
56,475	105,111 ± 1,404
69,025	100,971 ± 1,295
81,575	94,541 ± 0.430
	Mean: 99.26%; SD: 4.14
Relative uncertainty	3.63%