Table 2.
Flu‐6plx assay results in comparison with resolved data from monoplex PCRs for 213 respiratory samples
| Monoplex result | Flu‐6plx result | Flu‐6plx sensitivity | Flu‐6plx specificity | |
|---|---|---|---|---|
| Influenza A positive | 106 | 106* | 100 | 100 |
| Influenza B positive | 11 | 11 | 100 | 100 |
| hMPV positive | 20** | 16 | 80 | 100 |
| RSV positive | 24*** | 22 | 92 | 100 |
| Negative | 58 | 60 | ||
| Total tested | 213 | 213 | ||
| Single infections | 149 | 151 | ||
| Co‐infections | 6 | 2 |
*Includes four influenza A/H1 component positive, matrix component negative Flu‐6plx results. The Flu‐6plx assay identified subtype in these samples as 23 human H1, 42 2009 pandemic H1 and 41 presumptive H3.
**Low‐level hMPV RNA detected in four additional samples by monoplex PCR (Cp range, 35–41). Three were co‐infections with influenza A.
***Low‐level respiratory syncytial virus (RSV) RNA detected in two additional samples by monoplex PCR (Cp range, 33–37). One was a co‐infection with influenza A.