Table 3.

 Flu‐6plx assay influenza A subtyping results in comparison with ProFlu ST subtyping assay for 95 influenza A–PCR positive respiratory samples

	ProFlu ST result	Flu‐6plx result	Resolved subtype*	Flu‐6plx sensitivity	Flu‐6plx specificity
Influenza A subtype
Human A/H1	18	22	21	100	98
2009 pandemic A/H1	25	36	33	100	95
Human A/H3	35	ND	36
Untyped	17	37**	5***

*Influenza A subtype resolved using ProFlu ST test, CDC 2009 pandemic H1 PCR and QCMD data.

**Flu‐6plx assay does not identify influenza A/H3 subtype. These samples correspond to the 36 resolved influenza A/H3 samples plus another untyped low‐level influenza A RNA matrix PCR (Cp = 37).

***Five samples with unresolved subtypes all contained low‐level influenza A RNA (matrix PCR Cp > 34).

ND, not determined.