Fig 6. Gating strategy and overview of T_reg (CD4⁺/CD25⁺/FoxP3⁺) isolated from gMLN with and without HLA-G treatment after allogenic intestinal transplantation.

(A) Gating strategy and representative result for Treg analysis. (B) Overview of classical Treg (CD4⁺/CD25⁺/FoxP3⁺ Treg), isolated from gMLN. A tendency to higher abundance in HLA-G treated animals, without reaching statistical significance (Mann-Whitney U test p>0.05), was observed.