Figure 7.
The correlation between single radial haemolysis (SRH) and haemagglutination inhibition (HI) assays. Groups of five mice were intranasally immunised twice (21 days apart) with a subunit (SU) influenza A H5N1 vaccine. The control group consisted of unimmunised mice. Three groups were vaccinated with different antigen doses (7.5, 15 or 30 μg HA) of the chitosan‐adjuvanted SU vaccine. One group was vaccinated with a non‐adjuvanted SU vaccine with 30 μg HA, and a further group was immunised with a non‐adjuvanted 15 μg HA whole virus vaccine. The SRH results 3 weeks after the second immunisation are presented on the x‐axis as the geometric mean area in mm2, and the HI results measured 2 weeks after the second immunisation are presented as the HI titre on the y‐axis. HA, haemagglutinin.