Table 6. Primer sequences of the AS-PCR and the SNP loci.
Primer Name | Sequences of the Special Primers (5’-3’) | PCR Product | Original Fragment | ||
---|---|---|---|---|---|
Forward | Reverse1 | Reverse2 | |||
AS_1 | TCCCGTGGCTCTAACCTCT | TCTTGAACCATTTTTTTCCCT | TCTTGAACCATTTTTTTCCCC | 305 bp | M16337 |
AS_2 | TAGAACAAGACTCGGAAAA | CGCACATCGTACGTAAAA | CGCACATCGTACGTAAAG | 151 bp | M25207 |
AS_3 | CCAACGAGCAGCATCCAAAG | GGCGACTCGAACTTCTCCG | GGCGACTCGAACTTCTCCA | 348 bp | M38412 |