Table 10. Comparison of the results of sequencing and RT-PCR analyses of differentially expressed miRNAs.
Small RNA sequencing | RT-PCR | |||
---|---|---|---|---|
miRNA | Group | Log2(FC) | Log2(FC) | P value |
miRNA-143 | 1 | -0.131 | -1.120 | 0.022* |
2 | -0.032 | -0.305 | 0.690 | |
3 | -0.802 | -0.816 | 0.038* | |
let-7c | 1 | -1.189 | -1.181 | 0.041* |
2 | -0.687 | -0.720 | 0.426 | |
3 | -0.491 | -0.461 | 0.014* | |
NW_004080189.1_7961 | 1 | -1.436 | -1.142 | 0.047* |
2 | -0.778 | -0.347 | 0.855 | |
3 | -0.658 | -0.800 | 0.061 | |
NW_004080181.1_3961 | 1 | 0.008 | 0.011 | 0.001** |
2 | -1.995 | -2.251 | 0.002** | |
3 | 2.006 | 2.260 | <0.001** | |
NW_004080166.1_10417 | 1 | 0.270 | 0.310 | 0.002** |
2 | -2.055 | -1.841 | 0.020* | |
3 | 2.232 | 2.148 | <0.001** |
Note: 1, small and large waves; 2, small and medium waves; 3, medium and large waves