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. 2016 Jul 12;11(7):e0158599. doi: 10.1371/journal.pone.0158599

Fig 5. Hepcidin response to iron treatment depends on the diets.

Fig 5

WT mice were challenged with or without intraperitoneal BA injection and 14d later treated with or without intravenous iron. (A) Hepatic hepcidin mRNA levels 24h after iron treatment in mice fed a regular iron diet (black bars) or an iron deficient diet (white bars) (iron deficient diet: n = 4, 2-way ANOVA P = 0.006; *P = 0.01: PBS/PBS vs BA/iron; ***P = 0.0002: BA/PBS vs BA/iron). (B) Hepcidin mRNA levels 7d after the iron treatment in mice as in (A) (iron deficient diet: n = 4, 2-way ANOVA P = 0.0007; *p = 0.02: PBS/PBS vs BA/PBS; **P = 0.001: BA/PBS vs BA/iron). (C) Liver iron content (LIC) was determined in C57BL/6 mice fed a regular or iron deficient diet. LIC 14d after BA and 24h after intravenous iron administration are shown (regular diet: n = 3–4, 2-way ANOVA P = 0.002; **P = 0.008: PBS/PBS vs BA/PBS, iron deficient diet: n = 4, 2-way ANOVA, P < 0.0001; **P = 0.005: PBS/PBS vs BA/iron; **P = 0.004: BA/PBS vs BA/iron). (D) LIC 14d after BA and 7d after iron treatment in mice fed a regular or an iron deficient diet (iron deficient diet: n = 4, 2-way ANOVA P = 0.0002; *P = 0.01: PBS/PBS vs BA/iron; *P = 0.01: BA/PBS vs BA/iron).