Preparation of cells in ECM                                                     (6 mg/ml Bovine Collagen Type I + 10% BM)
178.8 µl	Cell culture grade water containing the desired number of 231 cells (determined above)
606 µl	Collagen I (10 mg/ml bovine), add drop by drop
100 µl	Basement membrane, thawed
100 µl	10x DMEM (containing phenol red) with the desired number of CAF (determined above)
15.2 µl	7.5% (v/v) Sodium Bicarbonate, add drop by drop