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. 2016 May 9;(111):54004. doi: 10.3791/54004
H&E Staining
Station Solution Time 
1 Xylene 5 min
2 Xylene 5 min
3 Xylene 5 min
4 100% Ethanol     5 min
5 100% Ethanol      5 min
6 95% Ethanol     5 min
7 95% Ethanol     5 min
8 Tap Water     5 min
9 De-ionized Water       5 min
10 Hematoxylin 7211 5 min
11 Tap Water          5 min
12 Clarifier* 10 dips
*Richard Allan #7401 or 70% Ethanol + 0.5% HCl
13 Tap Water                  5 min 
14 Bluing Reagent 30 sec
15 Tap Water 5 min
16 95% Ethanol 10 dips
17 Eosin-Y 1 min
18 95% Ethanol 10 dips
19 95% Ethanol 10 dips
20 100% Ethanol 10 dips 
21 100% Ethanol 10 dips
22 100% Ethanol 5 min
23 Xylene 10 dips 
24 Xylene 5 min