H&E Staining | ||
Station | Solution | Time |
1 | Xylene | 5 min |
2 | Xylene | 5 min |
3 | Xylene | 5 min |
4 | 100% Ethanol | 5 min |
5 | 100% Ethanol | 5 min |
6 | 95% Ethanol | 5 min |
7 | 95% Ethanol | 5 min |
8 | Tap Water | 5 min |
9 | De-ionized Water | 5 min |
10 | Hematoxylin 7211 | 5 min |
11 | Tap Water | 5 min |
12 | Clarifier* | 10 dips |
*Richard Allan #7401 or 70% Ethanol + 0.5% HCl | ||
13 | Tap Water | 5 min |
14 | Bluing Reagent | 30 sec |
15 | Tap Water | 5 min |
16 | 95% Ethanol | 10 dips |
17 | Eosin-Y | 1 min |
18 | 95% Ethanol | 10 dips |
19 | 95% Ethanol | 10 dips |
20 | 100% Ethanol | 10 dips |
21 | 100% Ethanol | 10 dips |
22 | 100% Ethanol | 5 min |
23 | Xylene | 10 dips |
24 | Xylene | 5 min |