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. 2016 Jul 12;11(7):e0158419. doi: 10.1371/journal.pone.0158419

Fig 5. JFH1Δ and Δ4 replication in Huh 7 cells.

Fig 5

(A, B) Huh7 cells were transfected with JFH1wt, JFH1Δ, JFH1Δ4 or no RNA (mock transfection control) and monitored for intracellular positive and minus sense HCV RNAs or minus-sense RNA alone by qRT-PCR. Data were expressed as copies per μg total RNA. Total intracellular HCV RNA was also monitored by northern blots as shown. GAPDH mRNA was analyzed as control. (C) Huh7 cells were infected with medium collected from HCV RNA transfected cells at times shown, and analyzed for HCV RNA after 48 hrs by qRT-PCR. Data were expressed as HCV RNA copies per μg total RNA. (D) JFH1wt versus JFH1Δ4-replicating cells were analyzed for core and F/ARFP proteins by immunofluorescence staining using indicated antibodies and quantified using ImageJ. Lines with P values also indicate statistically significant difference (P < 0.05) or no difference (P > 0.05) between samples.