Table 1. JFH1 constructs.

Location of nt. substitutions and anticipated effects on protein synthesis and SLV/VI structures are shown. +++ indicates normal or relatively high expression; + indicates reduced expression.

Construct	Mutation	Core	F/ARF	ARF/DS	Core+1/S	ARF/26(f+1)	SLV/VI
JFH1wt & Core	None	+++	+ (by -2/+1 frameshift)	Normal	Normal	Normal	Normal
JFH1Δ & CoreΔ	-2/+1 frame stops at codons 30, 31	+++	None (truncated at 29th codon)	Normal	Normal	None (truncated at 29th codon)	Base of SLVI altered
JFH1Δ4 & CoreΔ4	-2/+1 frame stop at codon 22	+++	None (truncated at 21st codon)	Normal	Normal	Normal	Normal (Loop of SLV altered)
F	1 nt. deletion at codon 10	+ (by -1/+2 frameshift)	+++	Normal	Normal	Normal	Normal
FΔ	1 nt. deletion at codon 10, & 0 frame stop at codon 29, 33	None (truncated at 28th codon)	+++	None (truncated at 28th codon)	Normal	Normal	Base of SLVI altered
HA-F	1 nt. deletion at codon 9, & 0 frame stop at codon 53	None (truncated at 52nd codon)	+++	Normal	Normal	Normal	Base of SLVI altered