Table 2. List of qRT-PCR primers.
Set | Gene | Direction | Sequence (5’ to 3’) |
---|---|---|---|
1 | JFH1 | Sense | TCTGCGGAACCGGTGAGTA |
Antisense | TCAGGCAGTACCACAAGGC | ||
2 | JFH1 | Sense | CGGGAGAGCCATAGTGG |
Antisense | AGTACCACAAGGCCTTTCG | ||
3 | IFNβ1 | Sense | CATTACCTGAAGGCCAAGGA |
Antisense | CAATTGTCCAGTCCCAGAGG | ||
4 | IFNβ1 | Sense | CCAACAAGTGTCTCCTCCAAA |
Antisense | CCTCAGGGATGTCAAAGTTCA | ||
5 | IFNα2 | Sense | TGAAAACTGGTTCAACATGG |
Antisense | TAATGGATCAGTCAGCATGG | ||
6 | IFNα21 | Sense | GCCCTGTCCTTTTCTTTACTG |
Antisense | TCCTTTGTGCTGAAGAGATTG | ||
7 | IFNα8 | Sense | CTTCAACCTCTTCAGCACAAA |
Antisense | AGGATGGAGTCCTCGTACATC | ||
8 | IFNλ1 | Sense | GCTGGTGACTTTGGTGCTA |
Antisense | GAGATTTGAACCTGCCAATGTG | ||
9 | IFNλ2/3 | Sense | CCACATAGCCCAGTTCAAGT |
Antisense | GCGACTCTTCTAAGGCATCTT | ||
10 | RIG-I | Sense | CTCTGCAGAAAGTGCAAAGC |
Antisense | GGCTTGGGATGTGGTCTACT | ||
11 | MDA-5 | Sense | GTTTGGCAGAAGGAAGTGTC |
Antisense | GCTCTTGCTGCCACATTCTC | ||
12 | ISG56 | Sense | GCTGATATCTGGGTGCCTAAGG |
Antisense | CTTGAGCCTCCTTGGGTTCG | ||
13 | TNFα | Sense | CCATGTTGTAGCAAACCCTCAA |
Antisense | GCTGGTTATCTCTCAGCTCCA | ||
14 | GAPDH | Sense | GGTGGTCTCCTCTGACTTCAA |
Antisense | GTTGCTGTAGCCAAATTCGTT |