. 2016 Jul 12;11(7):e0158419. doi: 10.1371/journal.pone.0158419

Table 3. Frameshift signal sequences in frameshift reporter constructs.

Set	Gene	Direction	Sequence (5’ to 3’)
1	pJFH1FS-Luc(-1/+2, 0, -2/+1)	Sense	AATTC^aaagctt^cAACCTCAAACAGACACCATGAGCACAAATCCT AAACCTCAAAGAAAAACCAAAAGAAACG^a
		Antisense	AATTC^aGTTTCTTTTGGTTTTTCTTTGAGGTTTAGGATTTGTGC TCATGGTGTCTGTTTGAGGTTaagctt^cG^a
2	pJFH1FS(-2/+1NC)^d	Sense	AATTC^aaagctt^cAACCTCAAACAGACACCATGAGCACAAATCCT AAACCTCAAAGAAAAACCAAAAGAAACGTAA^bG^a
		Antisense	AATTC^aTTAC^bGTTTCTTTTGGTTTTTCTTTGAGGTTTAGGATTT GTGCTCATGGTGTCTGTTTGAGGTTaagctt^cG^a
3	pJFH1FS(-1/+2NC)^d	Sense	AATTC^aaagctt^cAACCTCAAACAGACACCATGAGCACAAATCCT AAACCTCAAAGAAAAACCAAAAGAAACGATAA^bG^a
		Antisense	AATTC^aTTA^bTCGTTTCTTTTGGTTTTTCTTTGAGGTTTAGGATT TGTGCTCATGGTGTCTGTTTGAGGTTaagctt^cG^a

^a EcoRI sites used in the cloning are underlined.

^b Modified sequences are bolded.

^c Other restriction sites (HindIII) are shown in lower case letters.

^dNC denotes negative control.