Time (min)	Flow (ml/min)	%A	%B	%C	Comment
0	1.5	100	0	0	5 min isocratic segment
5	1.5	100	0	0	From 5 to 25 min, linear gradient from 0 to 17.4% MeOH
25	1.5	82.6	17.4	0
26	1.5	0	0	100	Washout of eluent A to avoid salt precipitation (see Note 14)
30	1.5	0	0	100
31	1.5	0	100	0	Washout of compounds still retained in the column
35	2.0	0	100	0
45	2.0	0	100	0
46	1.5	0	100	0
47	1.5	0	0	100	Washout of MeOH to avoid salt precipitation (see Note 14)
50	1.5	0	0	100
51	1.5	100	0	0	Re-equilibration of the column at 100% A for the next run
60	1.5	100	0	0