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. 2016 Jul;55(1):58–71. doi: 10.1165/rcmb.2015-0183OC

Figure 2.

Figure 2.

STAT6−/− mice are protected from developing pulmonary fibrosis. WT and STAT6−/− mice were exposed to chrysotile (100 μg) intratracheally. Lungs were extracted 21 days after exposure. (A) Masson’s trichrome from WT or STAT6−/− mice. Representative micrographs of one of six animals/genotype. Magnification, ×20. (B) Hydroxyproline was measured (n = 4); *P < 0.05. (C) Active transforming growth factor (TGF)-β in the BAL fluid was measured (n = 7); *P < 0.05. (D) Lung fibroblasts were isolated from WT mice and cultured in BAL fluid from WT or STAT6−/− mice. Collagen I mRNA expression was determined by real-time PCR and normalized to β-actin (n = 3); *P < 0.05. (E) Conditioned media were obtained from fibroblasts cultured as in D, and collagen protein expression was determined by immunoblot analysis. Representative of three replicates.