Figure 3.

STAT6 is required for jmjd3 gene expression. (A) Schematic of STAT6 binding sites on jmjd3 promoter. (B) Nuclear fractions were isolated from both WT and STAT6^−/− BMDMs. Immunoprecipitation of STAT6 was performed from the DNA–protein complex, and PCR amplification was performed using primers to detect STAT6 binding site on jmjd3 gene region 4. Histone 3 was a positive control, and IgG was a negative control. Control DNA was amplified using primers to detect mouse recurrent pregnancy loss (RPL30) intron 2 provided by the manufacturer (Cell Signaling, Boston, MA). Representative of three replicates. (C) WT and STAT6^−/− mice were exposed to chrysotile (100 μg) intratracheally. Alveolar macrophages were isolated after 21 days. Jmjd3 mRNA expression was determined by quantitative RT-PCR, and normalized to β-actin (n = 3); *P < 0.05. IP, intraperitoneal; Jmjd3, Jumonji domain containing-3.