Figure 2.

Characterization of sorted Lin⁻ EpCAM⁺ ATII cells. Shown are representative images from immunofluorescence microscopy of lung cells collected before magnetic enrichment and after sorting, cytospun, and stained for (A) Pro–SP-C, (B) CCSP, (C) cytokeratin-8, (D) major histocompatibility antigen (MHC) II, (E) alkaline phosphatase (AP), and (F) epithelial cadherin (E-cadherin). For quantitative analysis of stain-positive cells, representative histograms from flow cytometric analysis of lung cells collected pre-magnetic enrichment and post-sort are shown to the right. Percentages shown are representative of three to five independent experiments. Isotype controls are plotted in shaded gray; specific immunostains are shown in blue. In the case of AP staining, the shaded gray plot indicates unstained cells.