Figure 7.

TLR ligand stimulation–induced secretion of cytokines and chemokines by ATII cells purified from WT and lyn^−/− mice. Equal numbers of purified WT or lyn^−/− ATII cells were cultured on matrigel without or with TLR ligands, as described in Materials and Methods. Shown are the levels of macrophage chemoattractant protein (MCP)-1, keratinocyte-derived cytokine (KC), IL-6, granulocyte/macrophage colony–stimulating factor (GM-CSF), and regulated upon activation, normal T cell expressed and secreted (RANTES) measured in cell culture supernatant collected 24 h after no stimulation or stimulation either by (A) TLR3 ligand poly I:C (4.5 μg/ml), (B) TLR4 ligand LPS (0.5 μg/ml), (C) TLR5 ligand flagellin (5 μg/ml), or (D) TLR9 ligand CpG (0.5 μg/ml). Results shown are representative of four independent experiments. The values are plotted as mean (±SD); n = 4–8 per group. ns, not significant. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.